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adenovirus expression vector human dominant negative tcf4  (Vector Biolabs)


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    Vector Biolabs adenovirus expression vector human dominant negative tcf4
    Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of <t>TCF7L2/TCF4</t> isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.
    Adenovirus Expression Vector Human Dominant Negative Tcf4, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adenovirus+expression+vector+human+dominant+negative+tcf4/tcf4/pmc04855923-541-11-14
    Average 90 stars, based on 1 article reviews
    adenovirus expression vector human dominant negative tcf4 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis"

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI80205

    Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of TCF7L2/TCF4 isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.
    Figure Legend Snippet: Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of TCF7L2/TCF4 isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.

    Techniques Used: Isolation, Cell Culture, Western Blot, Transfection, Transformation Assay, Infection, Expressing, Dominant Negative Mutation

    In interzone progeny during development, hedgehog (HH) signaling induces the expression of TCF7L2 (and human TCF4) isoforms, including dominant negative isoforms. Increased expression of TCF7L2 protein isoforms limits signaling by β-catenin (β-cat), resulting in an inhibition of expression of FGF18, leading to ectopic cartilage formation. In adult chondrocytes, HH signaling activity induces cartilage degeneration. Expression of dnTCF7L2 and other TCF7L2 isoforms induces the expression of catabolic enzymes, including ADAMTS4 and MMP13, which are involved in cartilage degeneration as part of OA. Increasing β-catenin activity rescues hedgehog-induced ectopic cartilage formation and cartilage degradation, likely by restoring the balance between HH and β-catenin signaling.
    Figure Legend Snippet: In interzone progeny during development, hedgehog (HH) signaling induces the expression of TCF7L2 (and human TCF4) isoforms, including dominant negative isoforms. Increased expression of TCF7L2 protein isoforms limits signaling by β-catenin (β-cat), resulting in an inhibition of expression of FGF18, leading to ectopic cartilage formation. In adult chondrocytes, HH signaling activity induces cartilage degeneration. Expression of dnTCF7L2 and other TCF7L2 isoforms induces the expression of catabolic enzymes, including ADAMTS4 and MMP13, which are involved in cartilage degeneration as part of OA. Increasing β-catenin activity rescues hedgehog-induced ectopic cartilage formation and cartilage degradation, likely by restoring the balance between HH and β-catenin signaling.

    Techniques Used: Expressing, Dominant Negative Mutation, Inhibition, Activity Assay

    Related Articles

    Infection:

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis
    Article Snippet: .. Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium. ..

    Expressing:

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis
    Article Snippet: .. Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium. ..

    Plasmid Preparation:

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis
    Article Snippet: .. Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium. ..

    Dominant Negative Mutation:

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis
    Article Snippet: .. Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium. ..

    Control:

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis
    Article Snippet: .. Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium. ..



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    adenovirus expression vector human dominant negative tcf4  (Vector Biolabs)
    90
    Vector Biolabs adenovirus expression vector human dominant negative tcf4
    Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of <t>TCF7L2/TCF4</t> isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.
    Adenovirus Expression Vector Human Dominant Negative Tcf4, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adenovirus+expression+vector+human+dominant+negative+tcf4/tcf4/pmc04855923-541-11-14
    Average 90 stars, based on 1 article reviews
    adenovirus expression vector human dominant negative tcf4 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of TCF7L2/TCF4 isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.

    Journal: The Journal of Clinical Investigation

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis

    doi: 10.1172/JCI80205

    Figure Lengend Snippet: Primary mouse costal chondrocytes (A–D) or human knee chondrocytes (E–K) were isolated and cultured. Protein levels of TCF7L2/TCF4 isoforms were determined by immunoblotting (A and E), and RNA levels of Axin2 (B and G), Fgf18 (C and H), Gli1 (D), total TCF4 (F), MMP13 (I), MMP3 (J), and ADAMTS4 (K) were determined by qPCR relative to Actb (B–D) or HPRT (F–K). (A–D) Primary mouse chondrocytes were transfected with scrambled (DMSO or PM) or siRNA mix (PM + siRNA) that selectively targets all isoforms of Tcf7l2 and treated with DMSO or PM (10 μM) for 48 hours. (B–D) Bars represent mean ± SEM normalized to DMSO-treated cultures. Data were log transformed prior to analysis by repeated-measures ANOVA followed by Tukey’s post-hoc tests. a, significantly different (P < 0.05) compared with unlabeled bars. (E–K) Primary human chondrocytes were isolated and infected with adenoviral vectors expressing GFP or a dominant negative form of TCF4. (E–K) Bars represent mean ± SEM. Data were log transformed prior to analysis by paired t test. *P < 0.05 compared with GFP-infected cells. See also Supplemental Figure 4.

    Article Snippet: Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium.

    Techniques: Isolation, Cell Culture, Western Blot, Transfection, Transformation Assay, Infection, Expressing, Dominant Negative Mutation

    In interzone progeny during development, hedgehog (HH) signaling induces the expression of TCF7L2 (and human TCF4) isoforms, including dominant negative isoforms. Increased expression of TCF7L2 protein isoforms limits signaling by β-catenin (β-cat), resulting in an inhibition of expression of FGF18, leading to ectopic cartilage formation. In adult chondrocytes, HH signaling activity induces cartilage degeneration. Expression of dnTCF7L2 and other TCF7L2 isoforms induces the expression of catabolic enzymes, including ADAMTS4 and MMP13, which are involved in cartilage degeneration as part of OA. Increasing β-catenin activity rescues hedgehog-induced ectopic cartilage formation and cartilage degradation, likely by restoring the balance between HH and β-catenin signaling.

    Journal: The Journal of Clinical Investigation

    Article Title: Hedgehog inhibits β-catenin activity in synovial joint development and osteoarthritis

    doi: 10.1172/JCI80205

    Figure Lengend Snippet: In interzone progeny during development, hedgehog (HH) signaling induces the expression of TCF7L2 (and human TCF4) isoforms, including dominant negative isoforms. Increased expression of TCF7L2 protein isoforms limits signaling by β-catenin (β-cat), resulting in an inhibition of expression of FGF18, leading to ectopic cartilage formation. In adult chondrocytes, HH signaling activity induces cartilage degeneration. Expression of dnTCF7L2 and other TCF7L2 isoforms induces the expression of catabolic enzymes, including ADAMTS4 and MMP13, which are involved in cartilage degeneration as part of OA. Increasing β-catenin activity rescues hedgehog-induced ectopic cartilage formation and cartilage degradation, likely by restoring the balance between HH and β-catenin signaling.

    Article Snippet: Human chondrocytes were infected with an adenovirus expression vector for human dominant negative TCF4 (Vector Biolabs) or an adenovirus-GFP (control, Vector Biolabs) for 24 hours at 50 MOI and replaced in fresh medium.

    Techniques: Expressing, Dominant Negative Mutation, Inhibition, Activity Assay